Metodi analitici basati su cromatografia, spettrometria di massa e sensori

Settore ERC

Attività

Research topics in Analytical and Bioanalytical Chemistry cover a wide range of areas including analytical separations based research, innovation in materials for sample treatment devices and mass-spectrometry instrumentation, novel sensors and biosensors, and elemental speciation analysis.

Analytical separations based research is focused on developing chromatographic methods in combination with mass spectrometry with enhanced selectivity. In this field major objective of the group is the development and validation of methods based on hyphenated techniques, i.e. gas chromatography- and liquid chromatography-mass spectrometry, for food quality, authenticity and safety assessment, food packaging studies, trace analysis of environmental pollutants, clinical biomarkers and compounds of concern in forensic toxicology as well as for characterization of biofuels in bio-waste treatment plants. Mass spectrometry-based strategies for proteomics in food research and for biological and forensic applications as well as for metaproteomics in plant sciences are also devised.

The expertise of the research group of Analytical Chemistry in the study of molecular receptors as selective materials for novel miniaturized sample treatment techniques has been proved effective for the successful development of reliable and easy-to-use analytical devices. Research activity is further directed towards innovations on materials and devices for different types of ionization techniques in mass spectrometry.

The Analytical Chemistry research addresses many of the major challenges concerning the development and validation of chemical sensors and biosensors based on innovative nanobiocomposite materials for the determination of diagnostic biomarkers in serum and other physiological fluids, for food safety and authenticity assessment (e.g. detection of genetically modified organisms at trace levels) and for environmental control. 

Another issue of concern to the research group is method development and validation in the field of elemental speciation analysis encompassing chromatography, atomic spectroscopy, complementary elemental and molecular mass spectrometry and the understanding of the fundamentals that underscore their analytical characteristics.

Expertise in the use of environmental scanning electron microscopy is used to evaluate surface morphology for the characterization of materials and tissues, for biology research and applied to environmental and food samples with a focus on nanoparticle analysis.

The grouping uses a range of scientific skills from physical property measurements and state-of-the-art facilities and analytical techniques, spectroscopy and microscopy. Chemometrics, metrology and quality assurance in chemistry, involving method optimization and data handling, method validation, interlaboratory comparisons, etc – supports all research directions of Analytical Chemistry group where measurements are involved.

The Analytical Chemistry group works together with companies in various sectors offering cooperation for project developments in the frame of commissioned work. A wide range of services is also offered for carrying conformity assessment on products for compliance to existing technical regulations.

Prodotti della ricerca

Sol-gel coated ion sources for liquid chromatography-direct electron ionization mass spectrometry
N. Riboni, L. Magrini, F. Bianchi, M. Careri, A. Cappiello

Analytica Chimica Acta 978 (2017) 35-41          

Quantitative proteomics analysis of platelet-derived microparticles reveals distinct protein signatures when stimulated by different physiological agonist

M. Milioli, M. Ibáñez-Vea, S. Sidoli, G. Palmisano, M. Careri, M.R. Larsen

Journal of Proteomics 121 (2015) 56-66

An amperometric immunosensor for diagnosis of celiac disease based on covalent immobilization of open conformation tissue transglutaminase for determination of anti-tTG antibodies in human serum

M. Giannetto, M. Mattarozzi, E. Umiltà, A. Manfredi, S. Quaglia, M. Careri

Biosensors and Bioelectronics, 62 (2014) 325-330

ESEM-EDS-based analytical approach to assess nanoparticles for food safety and environmental control

M. Mattarozzi, F. Bianchi, M. Maffini, F. Vescovi, D. Catellani, M. Suman, M. Careri

Talanta, 196 (2019) 429-435

Combined hyaluronate-​based films loaded with pemetrexed and cisplatin for the treatment of malignant pleural mesothelioma: Preliminary evaluation in an orthotopic tumor recurrence model

F. Sonvico, S. Barbieri, P. Colombo, E. Barocelli, C. Mucchino, A.M. Cantoni, P.G. Petronini, M. Rusca, P. Carbognani, L. Ampollini

European Journal of Pharmaceutical Sciences 123 (2018) 89-97