TOOLS FOR THE DIAGNOSIS AND THE CURE OF DISEASES
DNA is the molecule essential for the continuation of life and the transmission of hereditary characteristics to offspring . In it are written "words" ( code for amino acids) consisting of molecular "letters" (DNA Bases ) . Our Laboratory aims to use the same code letters in artificial molecules not found in nature . These compounds are peptide nucleic acids, named PNA, which are polyamide analogs of DNA obtaind by synthesis, which we can design, produce and chemically modify using modern Organic Chemistry techniques; we use PNA to target DNA, RNA and to create 'unnatural' double helices useful in modern nanotechnology, and we are involved in a series of studies aimed to understanding the fundamental principles underlying the DNA recongition processes.
PNAs can be used to "read" the DNA or its product, RNA, but most importantly can show the ability to block the expression of DNA linked to pathological conditions such as cancer or genetic diseases. One frontier in this research is to provide new tools for gene-editing, i.e. to change and 'repair' the DNA . We also develop new methods for using these molecules in combination with modern Nanotechnology and with advanced sensory devices, and the combination of therapy and diagnostics (theranostics). All these tools are very important in themodern concept of 'Personalized Medicine'.
Our research is or has been linked to several International and national project, including:
RISE project Nano Oligomed (Hybrid Nanostructured Oligonucleotide Platforms for Biomedical Applications)
Our role is to incorporate PNAs into multifucntional organic and inorganic nanoparticles
ULTRAPLACAD-(ULTRAsensitive PLAsmonic devices for early CAncer Diagnosis)
We fabricated all the PNA probes used in the project for the detection of NRAS and KRAS mutations.
For an illustration of the project see also these YouTube videos:
Fortunati, Simone, Rozzi, Andrea, Curti, Federica, Giannetto, Marco, Corradini, Roberto, Careri, Maria (2019)
Novel amperometric genosensor based on peptide nucleic acid (PNA) probes immobilized on carbon nanotubes-screen printed electrodes for the determination of trace levels of non-amplified DNA in genetically modified (GM) soy.
Fabbri, Enrica, Tamanini, Anna, Jakova, Tiziana, Gasparello, Jessica, Manicardi, Alex, Corradini, Roberto, Sabbioni, Giuseppe, Finotti, Alessia, Borgatti, Monica, Lampronti, Ilaria, Munari, Silvia, Dechecchi, Maria Cristina, Cabrini, Giulio, Gambari, Roberto (2018)
A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.
Bertucci, Alessandro, Silvestrini, Simone, Corradini, Roberto, De Cola, Luisa (2018)
Loading of PNA and other molecular payloads on inorganic nanostructures for theranostics.
Manicardi, Alex, Gambari, Roberto, de Cola, Luisa, Corradini, Roberto (2018)
Preparation of anti-miR PNAs for drug development and nanomedicine.
Veerbeek, Janneke, Steen, Raymond, Vijselaar, Wouter, Frederik Rurup, W , Korom, Sasa, Rozzi, Andrea, Corradini, Roberto, Segerink, Loes, Jurriaan Huskens, And (2018)
Selective Functionalization with PNA of Silicon Nanowires on Silicon Oxide Substrates.
Giovanardi, F , Cucinotta, A , Rozzi, A , Corradini, R , Benabid, F , Vincetti, L (2018)
Protein detection using hollow-core tube lattice fibers.
Movilli, Jacopo, Rozzi, Andrea, Ricciardi, Roberto, Corradini, Roberto, Huskens, Jurriaan (2018)
Control of Probe Density at DNA Biosensor Surfaces Using Poly(l-lysine) with Appended Reactive Groups.
Verona, Massimiliano Donato, Verdolino, Vincenzo, Palazzesi, Ferruccio, Corradini, Roberto (2017)
Focus on PNA Flexibility and RNA Binding using Molecular Dynamics and Metadynamics.
Mejia-Ariza, Raquel, Rosselli, Jessica, Breukers, Christian, Manicardi, Alex, Terstappen, Leon W M M , Corradini, Roberto, Huskens, Jurriaan (2017)
DNA Detection by Flow Cytometry using PNA-Modified Metal-Organic Framework Particles.
Barozzi, M , Manicardi, A , Vannucci, A , Candiani, A , Sozzi, M , Konstantaki, M , Pissadakis, S , Corradini, R , Selleri, S , Cucinotta, A (2017)
Optical Fiber Sensors for Label-free DNA Detection.
Manicardi, Alex, Rozzi, Andrea, Korom, Saå¡a, Corradini, Roberto (2017)
Building on the peptide nucleic acid (PNA) scaffold: a biomolecular engineering approach.
Elskens, Joke, Manicardi, Alex, Costi, Valentina, Madder, Annemieke, Corradini, Roberto (2017)
Synthesis and improved cross-linking properties of c5-modified furan bearing pnas.
Manicardi, A , Gyssels, E , Corradini, R , Madder, A (2016)
Furan-PNA: A mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA.
Manicardi, Alex, Bertucci, Alessandro, Rozzi, Andrea, Corradini, Roberto (2016)
A Bifunctional Monomer for On-Resin Synthesis of Polyfunctional PNAs and Tailored Induced-Fit Switching Probes.
Prasetyanto, Eko Adi, Bertucci, Alessandro, Septiadi, Dedy, Corradini, Roberto, Castro-Hartmann, Pablo, De Cola, Luisa (2016)
Breakable Hybrid Organosilica Nanocapsules for Protein Delivery.
Brognara, Eleonora, Fabbri, Enrica, Montagner, Giulia, Gasparello, Jessica, Manicardi, Alex, Corradini, Roberto, Bianchi, Nicoletta, Finotti, Alessia, Breveglieri, Giulia, Borgatti, Monica, Lampronti, Ilaria, Milani, Roberta, Dechecchi, Maria Cristina, Cabrini, Giulio, Gambari, Roberto (2016)
High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222.
Alessandro Bertucci, Alex Manicardi, Alessandro Candiani, Sara Giannetti, Annamaria Cucinotta, Giuseppe Spoto, Maria Konstantaki, Stavros Pissadakis, Stefano Selleri, Roberto Corradini (2015)
Detection of unamplified genomic DNA by a PNA-based microstructured optical fiber (MOF) Bragg-grating optofluidic system.
Bertucci, Alessandro, Prasetyanto, Eko Adi, Septiadi, Dedy, Manicardi, Alex, Brognara, Eleonora, Gambari, Roberto, Corradini, Roberto, De Cola, Luisa (2015)
Combined Delivery of Temozolomide and Anti-miR221 PNA Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells.
Accetta, Alessandro, Petrovic, Ana G , Marchelli, Rosangela, Berova, Nina, Corradini, Roberto (2015)
Structural Studies on Porphyrin-PNA Conjugates in Parallel PNA:PNA Duplexes: Effect of Stacking Interactions on Helicity.
Montagner, Giulia, Gemmo, Chiara, Fabbri, Enrica, Manicardi, Alex, Accardo, Igea, Bianchi, Nicoletta, Finotti, Alessia, Breveglieri, Giulia, Salvatori, Francesca, Borgatti, Monica, Lampronti, Ilaria, Bresciani, Alberto, Altamura, Sergio, Corradini, Roberto, Gambari, Roberto (2015)
Peptide nucleic acids targeting β-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells.