Chimica Bioorganica ed Acidi Nucleici Artificiali

Settore ERC

Attività

Link Lab (SCVSA servizi)

ARTIFICIAL DNA

TOOLS FOR THE DIAGNOSIS AND THE CURE OF DISEASES

DNA is the molecule essential for the continuation of life and the transmission of hereditary characteristics to offspring . In it are written "words" ( code for amino acids) consisting of molecular "letters"  (DNA Bases ) . Our Laboratory aims to use the same code letters in artificial molecules not found in nature . These compounds are peptide nucleic acids, named PNA, which are polyamide analogs of DNA obtaind by synthesis, which we can design, produce and chemically modify using modern Organic Chemistry techniques; we use PNA to target DNA, RNA and to create 'unnatural' double helices useful in modern nanotechnology, and we are involved in a series of studies aimed to understanding the fundamental principles underlying the DNA recongition processes.

PNAs can be used to "read" the DNA or its product, RNA, but most importantly can show the ability to block the expression of DNA linked to pathological conditions such as cancer or genetic diseases. One frontier in this research is to provide new tools for gene-editing, i.e. to change and 'repair' the DNA . We also develop new methods for using these molecules in combination with modern Nanotechnology and with advanced sensory devices, and the combination of therapy and diagnostics (theranostics). All these tools are very important in themodern concept of 'Personalized Medicine'.

Our research is or has been linked to several International and national project, including:

RISE project Nano Oligomed (Hybrid Nanostructured Oligonucleotide Platforms for Biomedical Applications)

https://cordis.europa.eu/project/rcn/213007/factsheet/it

Our role is to incorporate PNAs into multifucntional organic and inorganic nanoparticles

 ULTRAPLACAD-(ULTRAsensitive PLAsmonic devices for early CAncer Diagnosis)

ULTRAPLACAD_FACT SHEET

We fabricated all the PNA probes used in the project for the detection of NRAS and KRAS mutations. 

For an illustration of the project see also these YouTube videos:

https://www.youtube.com/watch?v=88n3IRsWTm8 (English version)

https://www.youtube.com/watch?v=AaMT6XIYV5c (Italian version)

Prodotti della ricerca

Papi, C, Gasparello, J, Zurlo, M, Manicardi, A, Corradini, R, Cabrini, G, Gambari, R, & Finotti, A. (2022) Combined Treatment of Bronchial Epithelial Calu-3 Cells with Peptide Nucleic Acids Targeting miR-145-5p and miR-101-3p: Synergistic Enhancement of the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene, International Journal of Molecular Sciences, 23. doi: 10.3390/ijms23169348; iris: 11381/2932148

Neri, M, Kang, J, Zuidema, J. M, Gasparello, J, Finotti, A, Gambari, R, Sailor, M. J, Bertucci, A, & Corradini, R. (2022) Tuning the Loading and Release Properties of MicroRNA-Silencing Porous Silicon Nanoparticles by Using Chemically Diverse Peptide Nucleic Acid Payloads, ACS Biomaterials Science & Engineering, 8, 4123-4131. doi: 10.1021/acsbiomaterials.1c00431; iris: 11381/2903902

Curti, F, Fortunati, S, Knoll, W, Giannetto, M, Corradini, R, Bertucci, A, & Careri, M. (2022) A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein, ACS Applied Materials & Interfaces, 14, 19204-19211. doi: 10.1021/acsami.2c02405; iris: 11381/2931853

Gasparello, J, Papi, C, Zurlo, M, Gambari, L, Rozzi, A, Manicardi, A, Corradini, R, Gambari, R, & Finotti, A. (2022) Treatment of Human Glioblastoma U251 Cells with Sulforaphane and a Peptide Nucleic Acid (PNA) Targeting miR-15b-5p: Synergistic Effects on Induction of Apoptosis, Molecules, 27. doi: 10.3390/molecules27041299; iris: 11381/2922453

Fortunati, Simone, Rozzi, Andrea, Curti, Federica, Giannetto, Marco, Corradini, Roberto, Careri, Maria (2019)
Novel amperometric genosensor based on peptide nucleic acid (PNA) probes immobilized on carbon nanotubes-screen printed electrodes for the determination of trace levels of non-amplified DNA in genetically modified (GM) soy.
https://air.unipr.it/handle/11381/2854686

Fabbri, Enrica, Tamanini, Anna, Jakova, Tiziana, Gasparello, Jessica, Manicardi, Alex, Corradini, Roberto, Sabbioni, Giuseppe, Finotti, Alessia, Borgatti, Monica, Lampronti, Ilaria, Munari, Silvia, Dechecchi, Maria Cristina, Cabrini, Giulio, Gambari, Roberto (2018)
A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.
https://air.unipr.it/handle/11381/2836690

Bertucci, Alessandro, Silvestrini, Simone, Corradini, Roberto, De Cola, Luisa (2018)
Loading of PNA and other molecular payloads on inorganic nanostructures for theranostics.
https://air.unipr.it/handle/11381/2848673

Manicardi, Alex, Gambari, Roberto, de Cola, Luisa, Corradini, Roberto (2018)
Preparation of anti-miR PNAs for drug development and nanomedicine.
https://air.unipr.it/handle/11381/2848674

Veerbeek, Janneke, Steen, Raymond, Vijselaar, Wouter, Frederik Rurup, W , Korom, Sasa, Rozzi, Andrea, Corradini, Roberto, Segerink, Loes, Jurriaan Huskens, And (2018)
Selective Functionalization with PNA of Silicon Nanowires on Silicon Oxide Substrates.
https://air.unipr.it/handle/11381/2850581

Giovanardi, F , Cucinotta, A , Rozzi, A , Corradini, R , Benabid, F , Vincetti, L (2018)
Protein detection using hollow-core tube lattice fibers.
https://air.unipr.it/handle/11381/2852349

Movilli, Jacopo, Rozzi, Andrea, Ricciardi, Roberto, Corradini, Roberto, Huskens, Jurriaan (2018)
Control of Probe Density at DNA Biosensor Surfaces Using Poly(l-lysine) with Appended Reactive Groups.
https://air.unipr.it/handle/11381/2853434

Verona, Massimiliano Donato, Verdolino, Vincenzo, Palazzesi, Ferruccio, Corradini, Roberto (2017)
Focus on PNA Flexibility and RNA Binding using Molecular Dynamics and Metadynamics.
https://air.unipr.it/handle/11381/2822607

Mejia-Ariza, Raquel, Rosselli, Jessica, Breukers, Christian, Manicardi, Alex, Terstappen, Leon W M M , Corradini, Roberto, Huskens, Jurriaan (2017)
DNA Detection by Flow Cytometry using PNA-Modified Metal-Organic Framework Particles.
https://air.unipr.it/handle/11381/2822608

Barozzi, M , Manicardi, A , Vannucci, A , Candiani, A , Sozzi, M , Konstantaki, M , Pissadakis, S , Corradini, R , Selleri, S , Cucinotta, A (2017)
Optical Fiber Sensors for Label-free DNA Detection.
https://air.unipr.it/handle/11381/2818030

Manicardi, Alex, Rozzi, Andrea, Korom, Saå¡a, Corradini, Roberto (2017)
Building on the peptide nucleic acid (PNA) scaffold: a biomolecular engineering approach.
https://air.unipr.it/handle/11381/2832976

Elskens, Joke, Manicardi, Alex, Costi, Valentina, Madder, Annemieke, Corradini, Roberto (2017)
Synthesis and improved cross-linking properties of c5-modified furan bearing pnas.
https://air.unipr.it/handle/11381/2836691

Manicardi, A , Gyssels, E , Corradini, R , Madder, A (2016)
Furan-PNA: A mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA.
https://air.unipr.it/handle/11381/2808278

Manicardi, Alex, Bertucci, Alessandro, Rozzi, Andrea, Corradini, Roberto (2016)
A Bifunctional Monomer for On-Resin Synthesis of Polyfunctional PNAs and Tailored Induced-Fit Switching Probes.
https://air.unipr.it/handle/11381/2820273

Prasetyanto, Eko Adi, Bertucci, Alessandro, Septiadi, Dedy, Corradini, Roberto, Castro-Hartmann, Pablo, De Cola, Luisa (2016)
Breakable Hybrid Organosilica Nanocapsules for Protein Delivery.
https://air.unipr.it/handle/11381/2801329

Brognara, Eleonora, Fabbri, Enrica, Montagner, Giulia, Gasparello, Jessica, Manicardi, Alex, Corradini, Roberto, Bianchi, Nicoletta, Finotti, Alessia, Breveglieri, Giulia, Borgatti, Monica, Lampronti, Ilaria, Milani, Roberta, Dechecchi, Maria Cristina, Cabrini, Giulio, Gambari, Roberto (2016)
High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222.
https://air.unipr.it/handle/11381/2808282

Alessandro Bertucci, Alex Manicardi, Alessandro Candiani, Sara Giannetti, Annamaria Cucinotta, Giuseppe Spoto, Maria Konstantaki, Stavros Pissadakis, Stefano Selleri, Roberto Corradini (2015)
Detection of unamplified genomic DNA by a PNA-based microstructured optical fiber (MOF) Bragg-grating optofluidic system.
https://air.unipr.it/handle/11381/2748112

Bertucci, Alessandro, Prasetyanto, Eko Adi, Septiadi, Dedy, Manicardi, Alex, Brognara, Eleonora, Gambari, Roberto, Corradini, Roberto, De Cola, Luisa (2015)
Combined Delivery of Temozolomide and Anti-miR221 PNA Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells.
https://air.unipr.it/handle/11381/2797835

Accetta, Alessandro, Petrovic, Ana G , Marchelli, Rosangela, Berova, Nina, Corradini, Roberto (2015)
Structural Studies on Porphyrin-PNA Conjugates in Parallel PNA:PNA Duplexes: Effect of Stacking Interactions on Helicity.
https://air.unipr.it/handle/11381/2797837

Montagner, Giulia, Gemmo, Chiara, Fabbri, Enrica, Manicardi, Alex, Accardo, Igea, Bianchi, Nicoletta, Finotti, Alessia, Breveglieri, Giulia, Salvatori, Francesca, Borgatti, Monica, Lampronti, Ilaria, Bresciani, Alberto, Altamura, Sergio, Corradini, Roberto, Gambari, Roberto (2015)
Peptide nucleic acids targeting β-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells.
https://air.unipr.it/handle/11381/2787333